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#Alleleid mgb allele probe genotyping iso

The taq polymerase synthesises a complementary DNA strand using the PCR primers and template as a guide. At this point the fluorescent dye of the probe is quenched by the MGB-Eclipse quencher.įigure 1: Gene Expression analysis (click to enlarge)īy decreasing the temperature the PCR primers and MGB probe anneal to their specific target sequence. Allelic discrimination is achieved by the selective annealing of the MGB probes (Figure 2). The double-stranded DNA is denatured by raising the temperature. MGB Probes for gene expression analysis & SNP genotyping It is therefore possible to use shorter probes (down to 13 bases) in real-time PCR compared to conventional dual labelled probes. Probes containing a MGB molecule form an extremely stable duplex which gives the probe a significant increase of the melting temperature (T m).

MGB Eclipse is a registered trademark of Elitech Group.Īdvantage of the MGB molecule in real-time PCR Select your required dye-quencher combination: 5' ReporterĮurofins Genomics' MGB Probes are licensed for human in vitro diagnostics applications to provide information for individual patient management solely using 5' nuclease (Taqman-MGB) technology for research grade licensed products and IVD grade licensed products. Our range of fluorophore options (FAM, HEX, CY5, and Yakima Yellow® (EliTech Group) allows you to ensure compatibility with your instrument and more easily design multiplex assays.Įurofins Genomics MGB Probes incorporate a 5’ fluorescent dye and the 3’ Eclipse quencher (EQ) attached to the minor groove binder (MGB) molecule. These probe are ideal for genotyping qPCR assays.

#Alleleid mgb allele probe genotyping iso

We have combined our proven oligo manufacturing expertise and ISO 9001:2015, GLP & GMP certified production processes to deliver MGB Eclipse Probes.

Begin your testing sooner with faster, more reliable delivery.

Transition from research to clinical diagnostics while maintaining robust assay performance.

MGB Eclipse® probes are manufactured, which allows you to: Each amplified probe contains a unique tag sequence that is complementary to a sequence on the universal tag array. Choose an assay from one of more than 17 million predesigned assays, or customize your own using our Custom Assay Design Tool.Short probes that incorporate a minor groove binder (MGB) to be used in 5’ nuclease (TaqMan - MGB) technology for human IVD applications. The probes are subsequently released from the genomic DNA and those that have been covalently circularized in the correct allele/nucleotide reaction combinations are amplified using a universal PCR primer pair.

TaqMan Performance Guarantee* ensures the assay will work as described or we’ll replace itĮach TaqMan SNP Genotyping Assay includes two differentially labeled, allele-specific TaqMan MGB probes and a PCR primer pair that uniquely amplify and provide unmatched specificity for the allele of interest.

Gold-standard chemistry backed by more than 40,000 publications and over 300 patents.

Flexible formats, including single tubes 48-, 96-, and 384-well plates and OpenArray plates, to accommodate any throughput and number of targets and samples.

Functional quality testing with 20 gDNA samples for each human assay.

Robust design pipeline with 90+ parameters for optimal primer-probe combinations.

Minor Groove Binder (MGB) technology enables TaqMan probes to discriminate between highly homologous allele sequences.

Applied Biosystems TaqMan SNP Genotyping assays help you detect and distinguish SNPs accurately and efficiently, so you can better understand their associations and roles in gene function and disease.